Sabyasachi Sarkar
Professor Emeritus at IIESTS
Title: Nano carbons cross blood brain barrier with potential to entrap and release Alzheimer drug and other relevant molecules
Biography
Biography: Sabyasachi Sarkar
Abstract
Water soluble Carbon Nano Onions (wsCNO) (25-50 nm) are used to image the life cycle of Drosophila melanogaster.\r\nThe multi-layered wsCNO may be used in drug delivery. The Blood–Brain Barrier (BBB) regulates brain homeostasis\r\nand selectively permits the entry of necessary molecules to pass into the brain through tight junctions and enzymatic carriers.\r\nThis BBB is the greatest impediment preventing any diagnostic or therapeutic probe in combating neuronal disorders or the\r\ngrowth of a tumor inside the brain. Fluorescent wsCNO may be used as a Trojan horse to carry the drug, the drug on its own\r\nis a foreign body, may be impermeable to the brain. We report here the crossing of wsCNO through the BBB in the murine\r\nmodel of CADASIL as well as in GBM induced mice. Donepezil, an inhibitor of acetylcholinesterase, is entrapped by wsCNO\r\nin acidic Phosphate Buffer Saline (PBS) demonstrating its function as Trojan horse from which the drug is readily released\r\nat pH 7.4. The action of wsCNO as Trojan horse is due to its physiological pH dependent ‘open and closed sesame’ behavior\r\nwhere the spherical wsCNO opens its shape as flat sheet to engulf the drug and closing it. This lecture will also present that\r\nseveral relevant large molecules like tetraphenylporphyrin or ferromagnetic mixed iron oxide can be entrapped and released\r\nsimilarly using graphene oxide prepared by a simple low cost method in the size range 40-200 nm as drug cargo under similar\r\npH dependent action.